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DNA was isolated from mouse placentae using the DNeasy Tissue Kit (Qiagen).
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Recently, the differential methylation in imprinting control regions was reported in maternally BPA-exposed mouse embryos and placentas using pyrosequencing technology.
We have compared placenta-enriched genes to genes with putative functional significance in the placenta using the mouse phenotype data and human PTB association study data.
We set out to characterise imprinting of the KCNQ1 domain, analysing allelic expression of central, ubiquitously imprinted genes, and the flanking genes that are only imprinted in the mouse placenta, in the early human placenta using EB-TS cells as an in vitro model of human trophoblast precursors.
We extracted RNAs from mouse placenta and ovaries using Trizol (Invitrogen), and performed reverse-transcription with Superscript III (Invitrogen) and oligo-dT.
The location of angiogenin was studied by indirect immunolocalization on cryosections of human 8- to 9-week placentas, using two angiogenin-specific antibodies: a mouse monoclonal antibody (mANG) and a rabbit polyclonal antibody (pANG).
First, Booker carefully washes the placenta, "using my fingers to express any blood that I can get out of the placenta.
Immunohistochemistry was performed on samples from all 6 placentas used.
The two most comprehensive gene expression profiling studies related to the placenta used microarray analysis to characterize four different components of the human placenta in 76 individuals [ 10] and the mouse placenta over the whole course of pregnancy [ 11].
n refers to the number of placenta used (two vessels per placenta).
The protocol for isolating trophoblast MVM from rat placenta was adapted from that used previously in mouse placenta (40).
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