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This mouse protein is also known as myofibrillogenesis regulator 1.
The corresponding mouse protein is 97% identical with the human SAP30L.
We introduced S112A mutation, which in the mouse protein is equivalent to the aa 127 in human Yap1.
This result provides important evidence that the mouse protein is a potentially useful model for human Ebola virus interaction with human sinusoidal endothelial cells.
In fact, it has been shown that mouse protein is easily extracted from brain by high salt buffer, instead human parkin is only extracted with harsher buffers, especially in elderly.
Similar(54)
Polyclonal rabbit sera to the full-length mouse protein were raised against the corresponding GST fusion protein purified from bacteria E. coli BL21RP+.
Mouse and human ZFP57 contain three and six Cys2His2 ZFs respectively, two of which, the first and the second of the mouse protein being homologous to the third and fourth of the human protein.
Recruitment of ZFP57/KAP1 at the ICRs is mediated by ZFP57 recognition of a methylated TGCmetCGC hexanucleotide motif; the first and second Cys2His2 ZFs of the mouse protein are sufficient for the high affinity recognition of this DNA target site [6].
cDNA encoding residues 25 122 of the mouse protein was amplified from pcDNA3.Sho and inserted between the NdeI and BamHI sites of the pET-19b vector (Novagen) and expressed in E. coli BL21 DE3)/RIL (Stratagene).
To understand the defense systems of vertebrates against viruses, comprehensive knowledge of relationships between viral and mouse proteins is necessary.
No disease-associated mutations of γ-synuclein gene or obvious cases of idiopathic human γ-synucleinopathies have been reported so far and the amino acid similarity between human and mouse proteins is high.
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