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In addition, the mouse gene is expressed exclusively in taste cells, and variations in the gene sequence distinguish taster mice from nontaster mice.
Sequence similarities, domain organization of the deduced peptide, and expression analysis allow us to conclude that this newly cloned mouse gene is Muc6, i.e., the mouse ortholog of human MUC6.
If a mouse gene is expressed at a given stage, we assumed the same activity of the human homologue (Figure 8).
Autoregulation of the Hoxa4 mouse gene is essential for maintaining normal levels of its expression during development of the embryo [8].
As a result of this rearrangement, the Rtn4r mouse gene is located between the Prodh gene (∼40-kb proximal) and the Zdhhc8 gene (∼70-kb distal) (Fig. 2A), both of which have been implicated by human genetic studies as strong candidate susceptibility genes for schizophrenia [21] [23].
Each mouse gene is analyzed in turn.
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As I once wrote in a book called "The Mansion of Happiness," a study of the history of life and death, a mouse gene was the first gene ever cloned; the mouse genome was the first genome decoded.
Primers used for amplifying 3'-UTR of each mouse gene were listed in Table S5.
In 28 cases, the yeast gene was essential, and in 24 of these families (86%), the mouse gene was also essential.
The mouse gene was also responsive to IBMX at the early time points, whereas these effects were only observed at days 1 and 3 in human cells.
A shRNA non-targeting human and mouse gene was used as control (shControl; Sigma-Aldrich; SHC002).
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