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This study evaluated the in vitro effect of PHA degradation product 3-HB and its derivatives (collectively called 3-HB derivatives) on cell apoptosis and cytosolic Ca2+ concentration of mouse glial cells.
All these aspects were considered in our study as we further optimized the transduction efficiency of AAV2/6 for mouse glial cells.
Importantly, co-culturing human iPSC-derived neurons with mouse glial cells improved neuronal maturation [ 9, 10].
Primary mouse glial cells were obtained from 1 or 2-day-old wild type or ApoB-100 transgenic mice [ 12, 13].
Furthermore, Xiao et al. report that PHB has the potential as a neural protective agent and reduces the number of apoptotic mouse glial cells when compared to those exhibiting asynchronous growth [ 11].
In neurons derived from human iPSCs, spontaneous neurotransmission occurs between 3 and 6 weeks after differentiation [ 9, 10, 104] and requires co-culturing of iPSC-derived neurons with mouse glial cells.
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Up-regulation of SERPINF1/ Serpinf1 by corticosteriod analogues has been reported in a variety of cell types: HUVECs [ 228], primary human trabecular meshwork cells [ 229], human ARPE-19 cells [ 228], mouse 3T3-L1 cells [ 227], mouse Müller glial cells [ 220] and rat glioma cells [ 220].
To enhance BBB characteristics, primary mouse endothelial cells were co-cultured with primary mouse cerebral glial cells and pericytes [ 17].
Here, we set out to optimize AAV2/6-mediated transduction of mouse Müller glial cells.
AAV2/6 vectors transduced mouse Müller glial cells aligning the retinal blood vessels.
Here, we delivered the GFAP promoter and GFP transgene to mouse Müller glial cells aligning the major retinal blood vessels via intravitreal injection of AAV2/6-GFAP-GFP.
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