Sentence examples for mouse colony with from inspiring English sources

C57BL/6 (WT) mice were purchased from Charles River Laboratories and used to maintain the transgenic mouse colony, with WT mice in experiments being littermates of transgenic mice (Tg) expressing the human Trx1 (hTrx1) gene.

Retrospective examination of banked tissue from a now defunct YFP-16 mouse colony with very high YFP expression levels (∼7 times greater than those found in our standard YFP-16 colony) revealed more severe morphological abnormalities, suggesting a dose-dependent effect (Figure 3).

One founder animal with stable and heritable expression of the transgene could be obtained, which was subsequently used to generate a mouse colony with stable over-expression of the frataxin protein.

Overall, through the generation and characterization of a novel conditional-KO mouse colony with SC-specific deletion of JAM-C, the findings of this study provide greater insight into the expression and function of JAM-C in peripheral nerves.

DR3−/− mice and their age-matched DR3+/+ (WT) littermates (ages 6 12 weeks) were used in the experiments; these mice were derived from a mouse colony with heterozygous DR-3 expression that was founded from mice provided by Cancer Research UK (38).

Cadwell et al. previously established mouse colonies with hypomorphic Atg16L1 protein expression (Atg16L1HM), which leads to aberrant disorganised granule packaging and a reduction of granule number in Paneth cells, as well as defective autophagy in thymocytes.

We have sequenced the genomes of two littermates from the A vy mouse colony, one with a yellow coat and one with a pseudoagouti coat, and searched for differences between the two, both at the A vy locus, and genome-wide, and confirm that genetic differences are unlikely to be involved in the variable coat colour.

At the other end of the mammalian spectrum, mice colonies with increased incidence of liver tumours were found to be colonized with Helicobacter hepaticus [ 10, 11], for which strain ATCC 51449 has been genome sequenced [ 11].

We would like to thank Julie Oughton for flow sorting, Anne-Marie Girard for microarray processing, Yuichi Ono for the antibody, Cliff Pereira for help with statistical methods, Jack Loflin and Ben Hung-Ping Shih for help with mouse colony maintenance, and the Center for Genome Research and Biocomputing and Steve Giovannoni for critical infrastructural support.

The authors wish to acknowledge Dionne Swor, Megan Sribour and Jessica Pellegrino for technical support and assistance with the mouse colony, Ying-Jiun Chen for help with the generation of the targeting construct, and members of the Gogos and Karayiorgou labs for useful suggestions.

We thank S. Brunet, J. Dumond, A. Karaiskou, P.V. Jallepalli, S. Louvet-Vallée and R. Poulhe for discussions and helpful advice, O. Haccard for discussions and help with the mouse colony, and C. Rachez for help with cloning and support.