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The blots were probed with: monoclonal mouse antibody specific for Phospho-Stat1 Tyr701 Phospho-Stat1 Tyr701abbit anti-ISG15 antibody and monoCellal mouSignalingdy against β-actin (BD).
In these experiments, cells were labelled with a mouse antibody specific for ovine CD14 (clone CAM36A, VMRD, Pullman, USA) and stained with a fluorochrome-conjugated goat anti-mouse immunoglobulin antibody (Caltag-Invitrogen).
After incubation and a wash step, antibody binding was revealed using HRP-conjugated goat antibody specific for human Fc for Synagis Mab, a mouse antibody specific for HA-tag (Zymed laboratories) in combination with a HRP-conjugated rabbit antibody specific for mouse antibodies (Dako BV) for detection of Synagis Fab and 101F Fab.
Phosphorylated histone 2AX (γH2AX) was assessed by probing Western blots of whole cell lysates with a monoclonal mouse antibody specific for histone 2AX phosphorylated at Ser139.
By cloning the hypervariable domains of a mouse antibody specific for human DC-SIGN into human framework regions, we obtained a humanized antibody.
Similar(55)
Mouse antibodies specific to recombinant EDIII of each of the four dengue serotypes, captured on microtiter wells sensitized with anti-mouse immunoglobulin antibodies, were recognized specifically and with high efficiency by the biotinylated antigen in conjunction with streptavidin enzyme conjugate.
A panel of goat-anti mouse antibodies specific for total IgG, IgG1, IgG2a, IgG2b and IgG3, obtained from Southern Biotechnology Associates (Birmingham, AL) as peroxidase-conjugated antibodies was used as per the manufacturer's instructions.
Linear peptide mapping identified in the sera of Prnp−/− mice antibodies specific for several B-cell epitopes located in the N-terminal unstructured portion of mPrP but only one located between aa 83 and 112 (P4) in the sera of wt mice.
Biotinylated rat anti-mouse IgGκ (clone 187.1; our collection) or peroxidase-conjugated goat anti-mouse antibodies specific for IgM, IgG1 or IgG2a (Southern Biotech, Birmingham, AL, USA) were used as detecting antibodies.
Horseradish peroxidase conjugated goat antibodies to rabbit IgG (1 1000) and mouse IgG (1 1000, for mouse monoclonal antibody specific for β-actin) were used as second antibodies.
p53: D07 mouse monoclonal antibody specific for human p53 protein wild-type and mutant forms; NCL-p53-PHOS mouse monoclonal antibody that detects human p53 protein phosphorylated at Ser (both Novocastra, Newcastle, UK).
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