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Exact(5)
Crystals were mounted in nylon loops, cryo-protected in 25% glycerol containing reservoir solution and frozen in liquid nitrogen.
The crystals were flash-frozen in liquid nitrogen after addition of 15% (v/v) glycerol to the mother liquor, mounted in nylon loops and flash-cooled in a cold nitrogen-gas stream at 100 K using an Oxford Cryosystem with reservoir solution as the cryoprotectant.
Crystals were mounted in nylon loops and flash-frozen in liquid N2 in the mother liquor containing various cryoprotectants.
Crystals were mounted in nylon loops (Hampton Research, Aliso Viejo, CA, USA), cryoprotected in Paratone-N (Hampton Research) and flash-cooled by immersion in liquid nitrogen.
Crystals of the PhoQW104C-A128C PD were mounted in nylon loops (Hampton Research, Aliso Viejo, CA) and directly frozen in liquid nitrogen.
Similar(55)
Electrodes were mounted in a nylon cap.
The crystal was mounted in a nylon loop and flash-cooled to 100 K in a N2 cryostream (Cryojet, Oxford Instruments, Abington, Oxfordshire, UK).
Crystals were mounted in a nylon loop (Hampton Research, Aliso Viejo, CA) and flash frozen in a nitrogen gas stream at 100 K. Diffraction data were collected from one selected crystal at beam line X-12B of the Brookhaven National Laboratory with the wavelength set to 0.9000 Å and 1° oscillation for each frame (total 360 frames).
The W203Y mutant crystal was mounted in a nylon loop and cryocooled in liquid nitrogen without further cryoprotection.
Optical sectioning of the nylon membrane mounted in immersion oil was performed at 1 μm intervals, from 0 to 60 μm.
Each intestinal sheet (1 cm in length) without any visible Peyer's patch with a support of nylon gauze were mounted in modified Ussing chambers (Warner instruments, CA) with an exposed surface area of 0.25 cm2 and maintained at 37°C by water-jacketed reservoirs.
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