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It features two stages of isolation mounted in series.
The husking mechanism was composed of two identical husking wheels mounted in series.
The system is composed by two double-cone actuators mounted in series, each of them providing actuation of two DoF.
To reduce the fluctuations of the temperature of thermostatic control the heaters were mounted in series with additional resistance that would be utilized, upon achieving such notion of reduced fluctuations, thus helping to increase the accuracy of ±0.03°.
Temperature was controlled with a gravity driven perfusion (1 2 ml/min) cooled with a peltier device mounted in series with a resistive heater (CellMicroControls).
Coronal sections (14 µm) of brain from male mice were prepared using a sliding microtome (Leica, Germany) and mounted in series on eight slides, which were subsequently stored at −80°C.
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Dechorionated embryos were mounted in halocarbon oil (series 700; Halocarbon Products) on petriPERM dishes (Sigma).
The stained mycelium was washed thoroughly with MQ water and phosphate buffer in series, and was mounted in MQ water.
After demineralisation, the specimens were dehydrated through an ascending ethanol series and mounted in paraffin.
Thereafter, the sections were incubated 45 min with Texas red conjugated goat anti-rabbit antibody, followed by 2 min incubation with 4',6-diamino-2-phenyl indole (DAPI), dehydrated in ascending ethanol series and mounted in Fluorescence Mounting Solution (Dakocytomation).
Immunocytochemically labeled brains were arranged on poly-lyseine-coated coverslips, dehydrated in a graded glycerol series, and mounted in Hard-Set Vectashield (Vector Laboratories, Burlin game, CA) as described in Shafer et al. [11].
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