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On whole mount immunostaining of wild type C. elegans (Figure 3G and data not shown), all of the antibodies exhibited similar staining patterns, labeling structures (probably Golgi) around the nucleus and also stain the vulva [59].
Whole mount immunostaining of tadpoles with 12/101 antibody, which identifies skeletal muscle, showed a delay in myogenic differentiation and a decrease in the number of muscle fibers in mDUX tadpoles on the injected side, compared to the contralateral and uninjected controls (Fig. 4B).
Whole mount immunostaining of fly brains was performed according to standard procedures.
Whole mount immunostaining of 36 hpf Tsp4b-deficient embryos using anti-MHC (A, D, G ; green) and anti-Tsp4b (B, E, H ; red) and merged (C, F, I ).
This allowed us to perform whole mount immunostaining of these compact structures and to use the optical sectioning capability of confocal microscopy to analyse the distribution of several proteins of interest in the entire spheroids.
Similar(55)
On whole mount immunostain of wild type C. elegans (Figure 5B), a similar pattern was seen that had previously been reported for rough endoplasmic reticulum membrane proteins and endoplasmic reticulum proteins [66].
On whole mount immunostain of wild type C. elegans, an immunostain pattern was observed similar to that previously reported for mitochondrial staining [56], [57]: signal around most nuclei, speckles in mitotic germ cells, foci in the cytoplasm of embryonic cells, and elongated tubular morphology in body wall muscle (Figure 3E).
On whole mount immunostain of wild type C. elegans, an immunostain pattern was seen that had previously been reported with the polyclonal antibody and DLG-1-GFP transgenes [18], [19], [20], [21]: adherens junctions of hypodermis, gut, and pharynx (Figure 3A and data not shown).
On whole mount immunostain of C. elegans embryos, a pattern was seen which was similar to that reported for both a polyclonal antibody and TAC-1 GFP TAC-1 GFP4], [55]: staining at meiotic spindle poles, at centrosomes in one-cell embryos and in the cytoplasm (fusions4D and E).
On whole mount immunostains of wild type, a staining pattern was observed that was similar to what had been reported for the polyclonal antibodies and the GFP-construct [27], [28], [29]: all cells of early embryos, epidermal cells, spermatheca, pharynx, intestine, uterus, and nerve ring (Figure 3D and data not shown).
Motoneurons also extended their axons normally, as shown by embryo whole-mount immunostaining of neurofilament M (NF-M) at E12.5 (Figure 3C).
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