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For the whole mount analysis of 9.5 dpc, 10.5 dpc and 11.5 dpc hind-gut or genital ridges, the region encompassing the hind-gut or genital ridges of each embryo was dissected in 20% FCS medium and washed in PBS.
Results from whole mount analysis of the lungs are depicted in Fig. 6d.
Semi-thin sections and whole mount analysis of embryos and seedlings were carried out as previously described [ 4, 12, 54].
Semi-thin sections and whole mount analysis of embryos were carried out as previously described (Treml et al., 2005).
Whole mount analysis of ovules was done after fixing and clearing the inflorescence in methyl benzoate as described previously [ 38].
For this purpose, we performed whole mount analysis of #4 inguinal mammary glands in virgin, pregnant and lactating FVB Cd151+/+ and Cd151−/− mice.
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Whole-mount analysis of the mammary glands revealed 2 to 5 variously sized tumor masses arising preferentially from the ducts closest to the nipple.
Whole-mount analysis of mammary glands in grafted mice showed normal ducts and small terminal end buds, similar to those observed in pubertal unmanipulated control mice as well as normal development of the mammary gland during the second half of gestation (Figure 5).
By contrast, 2 dpf embryos have a simple, transparent integument, which makes them amenable to whole-mount analysis of gene expression patterns in the developing CNS.
However, whole-mount analysis of the glands at 45 weeks after transplantation revealed extensive ductal hyperplasia in both control and hormone-treated glands.
Whole-mount analysis of type II collagen expression was performed in the fli1a EGFP transgenic background as previously described (Flanagan-Steet et al., 2009).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com