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In contrast, an N-terminal deletion Sdf-1γ mutant lacking the signal peptide (encoded by pSdf1γ−151 450V5) predominantly localized to the nucleus in most transfected cells (Fig. 3A).
Expression of hFis1 alone triggered mitochondrial fragmentation in most transfected cells (93%), in agreement with a previous report (Yoon et al, 2003).
We previously demonstrated that, although transfected cells were not specifically RGCs, most transfected cells in the ganglion cell layer were RGCs.
Surprisingly, TDRD3 was found concentrated in cytoplasmic granule-like foci in most transfected cells (data not shown), which led us to hypothesize that these cellular foci might be resulting from transfection-induced stress.
As shown in Figure 6C and Supplementary Material, Figure S3B, we observed that the EGFP-MC-LT fusion protein distributed in a ring-like pattern in most transfected cells and was found to label a high fraction of melanosomes in both Oa1+/− and Oa1−/− melanocytes (Fig. 6C), as well as Oa1−/−LOA1SN and Oa1−/−LOA1Δ18SN melanocytes (Supplementary Material, Fig. S3B).
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In control, vector-transfected cells, most of the transfected cells (GFP+) were still proliferating (47% PCNA+ or 66% Tbr2+) (Fig. 7, E and F) and localized to SVZ/VZ.
Analysis of murine skin sections after DNA electroporation demonstrated that most of the transfected cells were distributed around the panniculus carnosus muscle layer and in the hypodermis.
When transfected into this cell line, wild-type and D225E GFAP formed filament networks in most of the transfected cells (results not shown).
Although most of the transfected cells are fibroblast-like synoviocytes, a smaller number of monocyte-like synovial cells were also transfected.
Interestingly, YFP-hLsg1 accumulates in the nucleus over an 8 h period, and at 5 h most of the transfected cells showed punctate labeling in the nucleus reminiscent of Cajal bodies.
In most vector-transfected cells MCAK localized either to spindle poles only (not shown) or to spindle poles and a few KTs (Figure 6B, vector).
Related(20)
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