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This is the most rigorous validation protocol.
The most rigorous validation is external validation, i.e., validation performed in an independent data set.
The default validation type was "compounds out", i.e., the most rigorous validation protocol.
PrecisionAb Antibodies are screened using whole-cell lysates from up to 12 different biologically relevant cell lines by far the industry's most rigorous validation process.
The concept of external validation is based on the insight that independent test data are required to assess the generalization performance of a model since prediction of unseen data is the most rigorous validation method [12],[32]-[36] ,[32]-[36]
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External model validation is considered to be the most rigorous method of model validation because the predictions of the established model are evaluated against a new dataset.
Most reviews do not elicit methodologies for conducting rigorous validation studies.
One of the most rigorous studies on CAP involved the derivation and validation of the Pneumonia Severity Index [ 3].
The experimental process of antibody validation is complex, with the most rigorous methods being comparison of wildtype vs a knockdown/knockout tissue and/or use of a second antibody to a different epitope.
This is the most rigorous control that you could ask for in terms of validation of the technique.
Most previously discovered candidate biomarkers in bench are still lacking rigorous validation, and only few biomarkers had gotten FDA approval in the US.
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