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The top 20 most frequent bulge and corresponding closing pair sequences found in the database are shown in Table 1.
For example, the most frequent bulge found in the database, 5′GAG3′, (Table 1) was not studied here.
The most frequent bulge sequence identified was 5′GAG3′ which accounts for 15% of all the trinucleotide bulges found in the database search.
The two most frequent bulge sequences in the database, 5′GAG3′ and 5′GAU3′, are similar and account for 15%and12%2%, respectively, of the bulge sequences found in the database.
Due to the ambiguity of the bulge sequence, this bulge was not studied; however, this study did investigate 7 of the top 10 most frequent bulge and corresponding closing pair sequences found in the database.
Similar(54)
Table 1 shows the top 20 most frequent trinucleotide bulge sequences found, which account for ∼86% of all of the trinucleotide bulges found in the database.
The most frequent occurring bulge and corresponding closing pair sequence is, which accounts for ∼12% of all of the bulges identified.
From analyzing the top 10 most frequent occurring trinucleotide bulge sequences from the database search (Table 1), bulges with a mix of purines and pyrimidines do not appear to be significantly favored over what would be statistically expected.
Considering the 16 duplexes in this study that were found in the database, the average free energy contribution of the bulge for the three most frequent was 2.8 ± 0.5 kcal/mol, while the average free energy contribution of the bulge for the three least frequent was 3.5 ± 0.5 kcal/mol.
It's also the most frequent churchgoers.
One of our most frequent homophone mixups.
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