Sentence examples for most efficient primer from inspiring English sources

From the 3 and 5 respective candidate NV GI and NV GII primer sets that were evaluated for efficiency using clinical samples, the most efficient primer sets were selected and used in this study (Table 3).

In addition to the three allele-specific markers, eleven forward and eleven reverse primers were designed to identify the most efficient primer pairs for the qPCR-based assay.

After designing several sets of primers using Primer3 (http://primer3.sourceforge.net/), we selected the most efficient primer pairs with the smallest and largest cycle thresholds (Cts) between the wild-type and variant signals.

Additional PCR reactions were performed and the most efficient primer pair for each sample type was determined: wx a 11 with wx a 8 for waxy samples, Wxonly7 with Wxonly2 for non- waxy samples and Wxcomn9 with Wxcomn6 for both types of samples.

As expected, dGMP incorporation was most efficient when the primer terminus contained dC opposite dG in the template.

The composition of the reaction mixture for the extension of immobilised primer was further improved by the addition of bovine serum albumin (BSA), a reported compound to counteract protein adsorption and inactivation on wafer surfaces.[ 38] Furthermore, numerous osmolytes were screened and 2-hydroxymethyl[18]crown-6 2-hydroxymethyl[18]crown-6 2-hydroxymethyl[18]crown-6rimer extension.

A 3 nm AuNP with low loading (1 1 AuNP DNA) was chosen to promote high efficiency of the aptamer binding to ATP, as observed in previous studies where hybridization of DNA on AuNP surfaces was most efficient at low primer loading levels and on surfaces of high curvature.

After evaluating several approaches, we found that two-round size selection purification is the most efficient way to remove primer dimer background (Additional file 1: Figure S1).

Amplification was most efficient using non-TIR targeting primers, with 67.74% and 54.18% of sequences that were amplified, respectively, with primer combinations 6 and 11, showing significant similarity to R-genes and RGAs.

In both cases, we show that the TPCR reaction is most efficient within a narrow range of primer concentrations.

By modifying the technique to be an isothermal reaction using the DNA polymerase Phi29, we reduced the error rate to 10%%, making primer extension the most efficient and cost-effective approach tested.