Sentence examples for most sensitive primer from inspiring English sources

The most sensitive primer set for NV GI was NKI-F/R/F2, which detected 22/24 positive samples (91.7%).

Based on their results, we used the most sensitive primer pairs 553F_W and 1334R_W, WspecF and WspecR, and fpbA F1 and R1.

Sometimes the PCR is not sensitive enough to detect the causing agent in clinical situations with expected low viral load or limited amount of sample, even if every measure has been taken to design the most sensitive primers and probe from the sequence known about a specific virus.

For studies that reported use of multiple PCR primers, only the three most sensitive primers were recorded.

Comparative studies have led to the recommendation of TBR primers [ 69] as the most sensitive primers for detecting T. evansi [ 70], and the Phenol-Chloroform method [ 71] as the most sensitive DNA preparation method [ 72].

The most sensitive of these primer pairs amplified a product of 61 bp from exon 7 of the Plxdc2 transcript and was subsequently used for calculation of Plxdc2 transcript levels (Forward primer 5'-CCAGTGAAAGTCGGGTTGTCTG-3'; reverse primer 5'-TGGGTATTTGCTGGATCCTGTG-3').

Noninfectious particles of a mutant of Rous sarcoma virus failed to exhibit DNA polymerase activity even with the use of the most sensitive synthetic template-primer complexes.

The m2a4 primer was the most sensitive and stable among the three.

However, it has been suggested that the technique using the TCZ1 and TCZ2 primers that amplify a 188 bp of a 195-bp repetitive nuclear sequence is the most sensitive compared with methods using S35 and S36 primers to amplify a 330-bp minicircle sequence [ 10, 19].

Attempts to amplify VP1/2A or VP1 regions of EV RNA detected in most clinical specimens failed despite the use of sensitive primer pairs that have been discussed recently (10 ).

Their results showed that real-time PCR with 16S-23S ITS primers and its probes was the most sensitive, indicating its potential for the diagnosis of human brucellosis in the clinical laboratory [ 41].