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IHC images were quantified using a semi-quantitative scale from 0 to 4, in which higher scores indicated more intense immunostaining.

Notably, in accordance with the results of Levidou et al. [36], our data found that T1-grade 1/2 tumors exhibited more intense immunostaining compared to T1/2-grade 3 tumors, suggesting that Cyclin D1 over-expression is more important during early stages of bladder carcinogenesis.

Ir-LBPsiRNA injection of the ticks also gave rise to more intense immunostaining of neutrophils around the bite site; since the antibody target in this assay is a membrane antigen, it is likely that yet another consequence of Ir-LBPsiRNA treatment of tIr-LBPsiRNA treatmentent degranulatiof following acticksisn of neutrophils.

The grade of OA tended to be less severe in animals given HA than in those give the vehicle only, and more intense immunostaining for glycosaminoglycans was observed with HA treatment compared with the vehicle [ 96].

HSPB1 protein expression levels of GBM cases with short and long survival were validated individually by western blot analysis, which showed more intense immunostaining of protein in GBM cases with short survival, confirming the possible usefulness of HSPB1 as predictive factor of worse prognosis (Fig.  3c).

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As shown in Figure  2D, the Huh-7 hepatoma cells infected with HBSP-Ad exhibited more intense immunostains than control cells, indicative of higher amount of BPDE-DNA adducts within the nuclei.

EAF2−/−VHL+/− animals displayed areas of more intense VEGF immunostaining of hepatocytes, particularly near thrombus formations (Fig.  7b).

While corneal epithelium was strongly positive for TRAIL expression with a more intense TRAIL immunostaining observed in the basal layers, conjunctival epithelium expressed TRAIL but at a lower intensity with respect to the corneal epithelium [ 28].

In primates, the hippocampus displays the strongest immunoreactivity, amygdala has a highly heterogeneous staining pattern (Côté et al. 1996) and ventral striatum displays more intense LSAMP immunostaining than the dorsal striatum (Côté et al. 1995).

In addition to samples with weakly stained epithelial tissue similar to normal ovaries, 64% of tumor samples showed a more intense epithelial HAS2-immunostaining.

As expected, disease markers were more severely affected in MPSIIIB mice at 8 months than at 3 months (figure 5B and E H): GM2 and GM3 ganglioside levels were higher, cell vacuolation was more prominent and ScMAS immunostaining was more intense.

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