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Some cytoplasmic regions demonstrate more intense fluorescence; this focal intensity may represent clusters or bundles of the conjugates.
Fiber surface typically exhibits more intense fluorescence.
In addition, AMP-QDs possessed a high PLQY, so they exhibited a more intense fluorescence in the cells than the widely used MPA-QDs under the same conditions (Fig. 3).
It follows from Fig. 5 that a much more intense fluorescence emission of HCPT was detected from all the four types of cells exposed to the NDs and the NRs than that of those exposed to NSs for the same sampling time.
As shown in Fig. 8, a much more intense fluorescence emission of HCPT was detected from the cells exposed to the HFNDs than that of those exposed to NDs after 8 h of incubation, illustrating that the FA on the surface of the particles could greatly enhance the cellular uptake.
Since a much more intense fluorescence signal of HCPT was detected from the HeLa cells exposed to the NDs than that of those exposed to free HCPT (See Additional file 1: Figure S7), the fluorescence in the HeLa cells was mainly caused by the internalization of the NDs, rather than that of HCPT.
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However, the gated stained cell populations displayed much more intense fluorescences in both windows (Fig. 3), which yielded an excellent signal-to-noise ratio.
Indeed, cells exhibit intense red fluorescence from JC-1 aggregates, and depolarized control cells exhibit very weak red fluorescence and more intense green fluorescence from JC-1 monomers.
On the contrary, GNP-NKCT1-treated cells showed more intense red fluorescence and reduced green fluorescence since apoptotic and necrotic cells could not exclude the dyes and gave a combination of orange red fluorescence.
More intense green fluorescence was observed with increasing time of incubation.
Similar results were obtained in isolated mitochondria from all sources (i.e., mitochondria exhibit more intense red fluorescence and higher red/green ratios than depolarized controls).
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more intense search
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