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It appears that European branch C5c1 is more differentiated, as far as two of three sequenced Polish mtDNAs formed a separate branch (C5c1a), defined by a coding region mutation at np 7694.
Surprisingly, the transplant growth began in TAMRA+ samples later than in the control, where both the cells capable of internalizing DNA and more differentiated as well as stromal cells were simultaneously present (Fig. 2B; Fig. S1).
Genetic profiles observed in this work showed some similarities between RS and SM samples, but epigenetic profiles were more differentiated as shown by PCAs (Figure 2) and differentiation indices (Table 3).
Vertebrates, from fishes through lizards to humans, develop in ways that are remarkably similar during early stages, but they become more and more differentiated as the embryos approach maturity.
In this system, both EC types grew vigorously for more than a week and became increasingly more differentiated, as shown by multiplexed gene expression.
LNCaP prostate cancer cells are generally considered less tumorigenic and more differentiated as compared to highly tumorigenic DU145 and PC3 prostate cancer cells.
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In addition, extremely low-frequency electromagnetic fields treatment induced a more differentiated phenotype as well as an increased expression in peroxisome proliferators-activated receptor isotype β, a class of transcription factors related to neuronal differentiation and cellular stress response.
In addition, there are technological developments, more transboundary cultural exchanges, facilitated by the freer trade of more differentiated products as well as by tourism and immigration, changes in the political landscape and ecological consequences.
One might also speculate that PDGF-AL stimulates expansion of a Gfap+ glial progenitor/stem cell population that in turn gives rise to progeny representing more differentiated astrocytic cells as well as oligodendrocyte progenitors.
As the low expressing cell lines show a more differentiated phenotype, as compared to the high expressing ones, it can be hypothesized that TGF-β mediated cancer EMT is accompanied with an increase of caveolin-1 expression.
As these spheroid cultures do not only contain CSCs, but also more differentiated cells, as shown previously by our group [ 26], we decided to compare the effect of dimerization of iCasp9 to treatment with 5-FU in a more specific test that allows to discriminate CSCs from their differentiated progeny.
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