However the authors should strengthen the validation of their results by more comparative analyses of data and tone down some of their conclusions.
The cause of such variation remains unclear and will necessitate more comparative analyses between the families with high deletion and those with low deletion rates.
The large number of transcripts without matches in these other plant species suggests that the Solanaceae contains unique sequences although this number may decrease as additional plant sequences become available in the future and more comparative analyses are performed.
Based on the current and previous analyses (Anderson and Bell 2009; Vaneckova et al. 2011), HIs based on mean daily temperature (such as HI01 HI03) may be the most useful and simplest metric for the United States, although more comparative analyses at the regional level are needed.
Thus, we cannot rule out that evolution of specific binding sites, such as those for the zinc-finger protein PRDM9 [ 24], is causing some of the variation and more comparative analyses at a fine scale (e.g. [ 7]) will be needed in the future.
This is one of the most difficult comparisons to evaluate, and as more comparative analyses are reported, the AGNC aims to develop proposals regarding how genes should be annotated when sequence and expression suggest contradictory findings about the descent of gene functionality.
