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This is probably due to in appropriate state of the charge in enzyme active site at the more alkaline condition.

These experiments were performed at pH 6.0 to diminish the massive autolysis that would occur under more alkaline condition.

Treatments with pH 4 resulted in the decrease of a more acidic condition of pH 3.56, while the treatment with pH 6 led to an increase of a more alkaline condition of around pH 8.5.

In this release study, HCPT-loaded nanoparticles showed a quicker release pattern in the more alkaline condition.

Typically, the substitution of Glu44 pushes the onset of the active site negative potential towards a more alkaline condition, increasing pH optima.

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Typically, the substitution of the residue Glu44 pushes the onset of the active site negative potential towards more alkaline conditions.

More alkaline conditions brought about by alkali hydroxides and the higher temperature of 60 °C accelerate the reaction.

Along the 100 ppm isopleth (Fig. 2 and 6, Table 4) conditions are oxidized and lead is predominantly in the form of chloride complexes under acid to mildly alkaline conditions (pH from 3 to approximately 7.5), while hydroxide complexes dominate lead speciation under more alkaline conditions (Table 4 and Fig. 6).

Along the 100 ppm isopleth conditions are oxidized (∑SO4 >> ∑H2S) with Pb and Zn predominantly in the form of chloride complexes under acid to mildly alkaline conditions (pH from 3 to approximately 7.5), while hydroxide complexes dominate Pb and Zn speciation under more alkaline conditions.

Moreover, more alkaline conditions can represent higher cellulose losses and larger process costs.

We observed an accelerated autoxidation of MPDP+/1.2-MPDP under more alkaline conditions (Fig. 5C, D), while more acidic conditions (e.g., close to mitochondria) stabilized MPDP+.

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