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When fused to various proteins, mfGFP monitored their localization in living cells.
In their experimental study, Londoño and Cadavid [ 8] injected mice intraperitoneally with labeled outer membrane lipoproteins of Borellia turicatae and monitored their localization in the brain.
Therefore, we generated stable, inducible cell lines expressing GFP-tagged PDGFRα-wt, −V561D, or -D842V receptors and monitored their localization in living cells using laser scanning microscopy.
To test whether Arg might interact with N-WASp to mediate these protrusions, we co-expressed Arg-YFP and mCherry-N-WASp in arg−/− fibroblasts and monitored their localization during fibroblast adhesion and spreading on fibronectin-coated coverslips.
To better understand how the age-associated protein deposit forms and what are its physiological constituents, we selected two proteins containing glutamine- and/or asparagine-rich domains, Sup35 and Dcp2, which are known to undergo conformational switches and to aggregate, and we monitored their localization in respect to the age-associated protein deposit.
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It can e.g. be used to facilitate the implantation of TEVG, to longitudinally monitor their localization and function, and to provide non-invasive and quantitative feedback on their remodeling and resorption.
As well as revealing genes, proteins and metabolites from those omic investigations, which relate to the basic structures of systems, it is important to have methods capable of monitoring their localizations, connections, and in particular their dynamics over time under various physiological or pathological conditions.
We generated GFP-fusions of full length hDDX41 and truncations of hDDX41 missing aa 153 622 and monitored their cellular localization after transfection in HEK293T cells.
To further explore the role of Numb in affecting the localization of aPKC and Par3 during EMT, we monitored their respective subcellular localization in a time-course of HGF treatment of subconfluent numb-shRNA cells.
To monitor their expression and subcellular localization, we generated plant expression constructs, where GFP was fused to the C-terminal end of SUBEX and SUBEX-C57Y and the resulting SUBEX-GFP and SUBEX-C57Y-GFP proteins were transiently expressed in N. benthamiana leaf epidermal cells by agrobacterial infiltration.
We show that transiently expressed membrane proteins can be directly monitored for their subcellular localizations by fluorescence microscopy.
Related(20)
observing their localization
investigated their localization
controlling their localization
determined their localization
monitored their learning
monitored their diversity
monitored their survival
monitored their labour
monitored their use
monitored their sleep
monitored their voice
monitored their performance
monitored their weight
monitored their reproduction
monitored their rehabilitation
monitored their progress
monitored their slackening
monitored their work
monitored their grid
monitored their gaze
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