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In this study, we have monitored the aggregation of green-fluorescent protein (GFP -tagged synphilin-1, as a rotenone-induced Parkinsonia-onset biomarker.
In this study, using dopaminergic SHSY-5Y cells, we have monitored the aggregation of green-fluorescent protein (GFP -tagged synphilin-1 (a known constituent of PD Lewy neurites) as a function of rotenone-induced nitrosative stress.
In order to provide insight into the mechanism of the process, we monitored the aggregation of a normal (AT3Q24) ataxin-3, an expanded (AT3Q55) ataxin-3, and the JD in isolation.
In the experiments, we monitored the aggregation of a defined number of cells consisting of equal number of each cell types.
Expression trials (results not shown) were performed to limit the aggregation of truncated protein and, following purification using immobilized metal affinity chromatography then ion-exchange chromatography, we monitored the aggregation state of the ΔN159ChlH protein using HLPC gel filtration.
We therefore studied the aggregation of α-synuclein in the presence and absence of 100 μ m lacmoid and monitored the aggregation by using ThT and the intrinsic fluorescence assay.
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The fluorescence probe technique using pyrene has also been employed to monitor the aggregation.
We monitor the aggregation process with quasi-elastic light scattering (QELS), zeta potential, and conductivity measurements at different salt concentrations.
In addition to the popular use of DLS in sizing individual MNPs, this analytical technique is also being employed to monitor the aggregation behavior of MNPs and the size of final clusters formed [55, 73].
Therefore, the use of DLS to monitor the aggregation kinetic of MNPs is important to provide direct feedback about the time scale associated with this process [55, 77]. Figure 8 illustrates the aggregation behavior of three species of 40-nm reactive nanoscale iron particles (RNIP), 27.5-nm magnetite (Fe3O4) MNP, and 40-nm hematite (α-Fe2O3) MNP [73].
However, QDAβ is a useful nanoprobe, if used as a small fraction of the unmodified Abeta, for monitoring the aggregation under the microscope.
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