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Therefore, a cheap, efficient and quantitative method capable of monitoring the aggregation from monomers to any HrMW species is highly desirable [13].
However, QDAβ is a useful nanoprobe, if used as a small fraction of the unmodified Abeta, for monitoring the aggregation under the microscope.
The thermal denaturation of unmodified and PEGylated ConA in free solution was tracked by monitoring the aggregation in a physiologically relevant buffer with dynamic light scattering (DLS).
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The fluorescence probe technique using pyrene has also been employed to monitor the aggregation.
We monitor the aggregation process with quasi-elastic light scattering (QELS), zeta potential, and conductivity measurements at different salt concentrations.
In this study, we have monitored the aggregation of green-fluorescent protein (GFP -tagged synphilin-1, as a rotenone-induced Parkinsonia-onset biomarker.
In this study, using dopaminergic SHSY-5Y cells, we have monitored the aggregation of green-fluorescent protein (GFP -tagged synphilin-1 (a known constituent of PD Lewy neurites) as a function of rotenone-induced nitrosative stress.
In addition to the popular use of DLS in sizing individual MNPs, this analytical technique is also being employed to monitor the aggregation behavior of MNPs and the size of final clusters formed [55, 73].
Therefore, the use of DLS to monitor the aggregation kinetic of MNPs is important to provide direct feedback about the time scale associated with this process [55, 77]. Figure 8 illustrates the aggregation behavior of three species of 40-nm reactive nanoscale iron particles (RNIP), 27.5-nm magnetite (Fe3O4) MNP, and 40-nm hematite (α-Fe2O3) MNP [73].
In order to provide insight into the mechanism of the process, we monitored the aggregation of a normal (AT3Q24) ataxin-3, an expanded (AT3Q55) ataxin-3, and the JD in isolation.
As such, in order to monitor the aggregation process by NMR HSQC experiments, we lowered the V22-SH3 concentration down to ∼50 µM and subsequently collected a series of HSQC spectra by gradually increasing the NaCl concentrations.
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