Sentence examples for monitor ligand binding from inspiring English sources

The pair-wise atomic distances used to monitor ligand binding geometries are either between the PhC quaternary ammonium nitrogen and specific binding site tryptophan residue side-chains (W47, W58 from PDC109/a and W93, W106 from PDC109/b) or between the PhC phosphate anionic oxygens and specific binding site tyrosine residues (Y30, Y54, Y60 from PDC109/a and Y75, Y100, Y108 from PDC109/b).

Two-dimensional N, H-HSQC experiments to monitor ligand binding were recorded using a 700 MHz instrument.

Standard UV/vis spectra of several CYP isoforms, in the wavelength range typically used to monitor ligand binding, are shown in Figure 1.

With a judiciously placed spin label, the EPR spectrum can serve to monitor ligand binding via associated shifts in conformational equilibria.

Alternatively, GFP variants have been conjugated to the N- and C-termini of periplasmic-binding proteins in order to monitor ligand binding by fluorescence energy transfer (Fehr et al. 2002; Fehr et al. 2003; Schultz and Moini 2003).

We reasoned that STD-NMR a could constitute a simple and efficient way of monitoring ligand binding at different pH values.

To determine the binding affinity of benzimidazole to the cryptic binding site, we monitored ligand binding through the saturable perturbation of the CcP heme Soret band.[ 16b] In the CcP-ga cavity site, benzimidazole can occupy multiple sites and has low micromolar affinity for the primary binding site.

If this hypothesis is correct, then FCS analysis provides a very powerful approach to monitor ligand-binding kinetics in real time and within discrete membrane microdomains of single living cells.

Mutation of ligand-binding residues within StAR START reduced this activity, consistent with the yeast assay monitoring ligand-binding.

Fluorescence anisotropy (FA) is one of the homogeneous fluorescence-based assays that enable continuous online monitoring of ligand binding kinetics.

Electrochemical real-time monitoring of ligand binding to an engineered opioid receptor specific for morphine is reported.