Sentence examples for monitored ligand binding from inspiring English sources

To determine the binding affinity of benzimidazole to the cryptic binding site, we monitored ligand binding through the saturable perturbation of the CcP heme Soret band.[ 16b] In the CcP-ga cavity site, benzimidazole can occupy multiple sites and has low micromolar affinity for the primary binding site.

By monitoring ligand binding characteristics and receptor-mediated stimulation of adenylyl cyclase, we have determined that the C-terminal portion of the third cytoplasmic loop and the N-terminal segment of the cytoplasmic tail appear to be critical for productive receptor-coupling to G proteins.

The pair-wise atomic distances used to monitor ligand binding geometries are either between the PhC quaternary ammonium nitrogen and specific binding site tryptophan residue side-chains (W47, W58 from PDC109/a and W93, W106 from PDC109/b) or between the PhC phosphate anionic oxygens and specific binding site tyrosine residues (Y30, Y54, Y60 from PDC109/a and Y75, Y100, Y108 from PDC109/b).

Two-dimensional N, H-HSQC experiments to monitor ligand binding were recorded using a 700 MHz instrument.

We reasoned that STD-NMR a could constitute a simple and efficient way of monitoring ligand binding at different pH values.

Standard UV/vis spectra of several CYP isoforms, in the wavelength range typically used to monitor ligand binding, are shown in Figure 1.

With a judiciously placed spin label, the EPR spectrum can serve to monitor ligand binding via associated shifts in conformational equilibria.

Alternatively, GFP variants have been conjugated to the N- and C-termini of periplasmic-binding proteins in order to monitor ligand binding by fluorescence energy transfer (Fehr et al. 2002; Fehr et al. 2003; Schultz and Moini 2003).

If this hypothesis is correct, then FCS analysis provides a very powerful approach to monitor ligand-binding kinetics in real time and within discrete membrane microdomains of single living cells.

Mutation of ligand-binding residues within StAR START reduced this activity, consistent with the yeast assay monitoring ligand-binding.

Time series of distance profiles monitoring PhC ligand binding during the MD simulations for the PDC109-PhC complex are shown in Fig. 3.