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Thus, MBRT can be used to monitor contamination of a fermentation process without making plate count.
Findings from this study can be used for the development of more efficient environmental sensors to monitor contamination of ground water with trace amounts of radioactive materials.
A water control tube containing all reagents except c-DNA was also included in each batch of PCR assays to monitor contamination of genomic DNA in the PCR reagents.
Appropriate controls for RT-qPCR experiments include no template controls (NTCs) and Reverse Transcriptase minus controls (RT-) which monitor contamination of reagents with amplicon and genomic DNA background respectively.
Control reactions were performed using the same procedures, but without RT to monitor DNA contamination in the RNA preparations and without RNA added to monitor contamination of the PCR reaction.
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Pesticides are used the world over; and, even though precautionary measures (such as using protective clothing and respirators, monitoring contamination of equipment and clothing, keeping workers out of recently sprayed areas, and requiring workers to wash thoroughly after exposure) can be instituted, poisoning not infrequently occurs in agricultural communities.
We have previously referred to the potential of such molecular markers in the context of monitoring contamination of organisms [36].
To monitor contamination, 1 negative control of PBS was included for every 5 samples in each run of DNA extraction and the ensuing PCR.
This suggests that detection of the specific electrochemical signature of hPSCs would be a valid approach to monitor potential contamination of undifferentiated hPSCs, which can assess the risk of teratoma formation efficiently and economically.
To monitor potential contamination of reagents, negative control reactions, in which water was used in place of cDNA, were included with each batch of PCR analysis.
To prevent and monitor the contamination of the samples during the laboratory processes, pre-PCR and post-PCR activities were carried out in different laboratories and negative controls were included in each batch of extraction and amplification [ 52, 53].
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