Ai Feedback
Exact(5)
Propidium iodine (PI, Sigma, Shanghai, China) staining was used to monitor cell membrane integrity.
The finding that SREBP processing is controlled by different lipids in mammals and flies (sterols and phosphatidylethanolamine, respectively) suggests that an essential function of SREBP is to monitor cell membrane composition and to adjust lipid synthesis accordingly.
To monitor cell membrane permeabilization we expressed the fluorophore CFP together with HKx-YFP.
Additionally, given that one essential function of SREBP appears to be its ability to monitor cell membrane composition and to adjust lipid synthesis accordingly [56], it is possible that the interaction of macrolides with the membrane provides a strong signal to the cell to compensate for membrane disruptions.
Cell permeability to propidium iodide was used to monitor cell membrane integrity as an indicator of apoptosis.
Similar(55)
PI (5 ng/ml) was added to the medium for monitoring cell membrane integrity.
PI (5 ng/ml) was added to medium for monitoring cell-membrane integrity.
We monitored whole cell membrane capacitance transients (Cm) induced by the activation of Cav1.2 subunits [α11.2 (dN60-del1773), α2δ and β2A] without and with SNAP-25, Sx 1A and synaptotagmin (SytI) (excitosome complex) expressed in Xenopus oocytes, as previously described [29] (Fig. 1A).
In the first assay, cell subsets were identified using fluorescently labeled antibodies, and viability was determined by staining with 50 μg/mL of propidium iodide (PI) to monitor losses in cell membrane integrity.
Thus, monitoring ATPase activity in cell membrane preparations or in purified membrane proteins allows for identification of those compounds that interact with the drug efflux transporters.
Thus, monitoring ATPase activity in cell membrane preparations, or purified membrane proteins, represents a method of identifying those compounds that interact with the drug efflux transporters.
Related(20)
control cell membrane
monitor cell wall
monitor cell surface
controls cell membrane
monitor sensor membrane
monitor cell uptake
monitor cell microenvironment
monitor cell state
monitor cell respiration
monitor surface membrane
monitor cell size
monitor cell responsiveness
monitor cell dissociation
monitor cell fate
monitor cell density
monitor cell cycle
monitor cell index
monitor cell biology
monitor cell phone
monitor cell repair
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com