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The design of target molecules was based on earlier results dealing with ABCG2 inhibition with flavone and chromone derivatives.

The alignment of the molecules was based on the conformation of a structurally related ligand bound to the αIIBβ3 and αvβ3 integrins in X-ray crystallographic studies.

The first method of alignment of the molecules was based on the binding information sourced from the crystallographic study, from which CoMFA Model 1 was derived.

This is in agreement with Dalgleish and Hunt (1995) who reported that the binding of cations on protein molecules was based on the formation of a short-range hydration repulsion.

Ligation between two ubiquitin molecules was based on the established protocol (Pickart and Raasi, 2005).

Identification of the assayed molecules was based on bead color and fluorescence, using fluorescently labeled reporter molecules associated with each target protein.

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Another method applied to increase the fluorescence of molecules is based on applying dielectric nanospheres [7].

The description of both proteins and molecules is based on the NAOMI model, meaning that consistent atom type and bond order information is available throughout the next steps.

Dalgleish and Hunt (1995) reported that the binding of cations on protein molecules is based on the formation of a short-range hydration repulsion.

Electric manipulation of polar molecules is based on the Stark shift of quantum levels in the presence of an electric field [40].

Furthermore, several commercial reagents used for mild and selective fluorination of organic molecules are based on the known reactivity of S-F groups.

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