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QMSP used for analysis of the methylation status of the candidate genes was based on the principle of fluorescence-based real-time PCR.
Selection of these genes was based on their identification from two or more expression-based genotypic comparisons involving 10-day and 25-day Golden Promise-Maythorpe comparisons and the Golden Promise-Morex comparisons.
Protein-protein interactions (PPI) prediction of differentially expressed genes was based on the STRING database.
Selection of the endogenous control genes was based on previous research investigating gene expression in neutrophils.
The selection of candidate genes was based on Gene Ontology (GO) biological process annotations (http://geneontology.org/page/download-annotations).
Selection of the reference genes was based on the microarray results using an algorithm described in Popovici et al. (2009).
Third, our selection of genes was based on the study of Padmos et al. (Padmos et al. 2008) which found these specific signature genes, possibly ruling out other important genes.
The SFPs in the cellulose synthase 1 and dolichyl-diphospho-oligosaccharide genes was based on a SNP, whereas the SFP in the LysM gene was due to a 13-bp indel (Fig. 5a, b).
(C) Predicted protein-protein interactions (PPI) analysis of differentially expressed genes was based on the STRING database, according to e-value = 1 × 10−10 and string score > 700, differentially expressing genes with interaction frequency > 80 were illustrated on the picture.
The relative expression of the selected genes was based on gene expression CT difference formula [40].
Additional assignment of orthology between fly and mouse genes was based on experimental data.
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