Sentence examples for molecules per centromere from inspiring English sources

Left y-axis represents centromeric CENP-A levels normalized to RPE cells; right y-axis shows number of CENP-A molecules per centromere (CEN).

Stochastic fluctuation measurements in CAY/− and CAY/−+OE cells indicates that wild-type cells contain ≥188 and ≥149 CENP-A molecules per centromere, respectively.

Combined, we find a similar number of CENP-A molecules per centromere when analyzing the nuclear pool (492 molecules; Figure 4C) as when measuring total cellular CENP-A.

In contrast, the use of C-terminally tagged Cse4 yielded estimates ranging from 1 to 8 Cse4 molecules per centromere (Joglekar et al., 2006; Coffman et al., 2011; Lawrimore et al., 2011; Shivaraju et al., 2012; Aravamudhan et al., 2013).

2) We are slightly uncomfortable with the apparent precision of the photometrics results arguing for a maximum of 2 Cse4 molecules per centromere because the TetR-GFP images have such a high nuclear background and thus require major image processing to extract the array signal.

" Given the fact that the estimate given in their study is more than an order of magnitude lower than the 400 CENP-A molecules per centromere that we find, that the measurements were made in a cell line from a different organism, and the admitted experimental limitations of their method, we do not feel that it is appropriate to cite this estimate as similar to ours in this section of the manuscript.

Moreover, live cell microscopy of GFP-tagged Cse4 have variously indicated that the number of Cse4 molecules associated with centromeres may be either several-fold greater than the two Cse4 molecules within a nucleosome (Coffman et al., 2011; Lawrimore et al., 2011), or oscillate during mitosis from one to two molecules per centromeric nucleosome (Shivaraju et al., 2012).

This provides confidence that 400 molecules of CENP-A per centromere in wild-type RPE cells is an accurate measure.

Correcting the amount of CENP-A measured in CAG/− cells for wild-type levels results in ≥377 molecules of CENP-A per centromere.

Because the centromeric fraction of CENP-A is fixed, we can calculate the absolute amount of CENP-A per centromere in our cell lines and show that wild-type RPE cells contain ∼400 molecules of CENP-A on an average centromere.

(G ) Calculation of average CENP-A copy number per centromere (CEN) in wild-type RPE cells.