In control cells, the read-through products were expressed at 42±3 molecules per cell for +6/+196 RNA, 24±4 molecules per cell for +6/+418 RNA, 7.3±2.1 molecules per cell for +6/+658 RNA and 2.1±0.6 molecules per cell for +6/+911 RNA (Supplementary Fig. 2c).
For reference, +21/+146 RNA, largely representing mature U1 snRNA, was present at 6.8±1.1 × 104 molecules per cell.
This is equivalent to approximately 360,000 molecules per cell, assuming a cell dry weight of 0.3 picograms.
(b) Correlation (log10 transformed mean of molecules per cell per gene) between hCS-MEF and each of the hCS-FF cultures or the hSS-MEF.
Fluorescence measured by microscopy or flow cytometry was directly proportional to protein concentration measured by quantitative immunoblotting". P.312 middle column 2nd paragraph: "Global cytoplasmic concentrations of cytokinesis proteins (Table 1) range from 0.04 μM formin Cdc12p (600 molecules per cell) to 63.2 μM actin (1.43 million molecules per cell).
On the other hand, transcripts upstream of the known transcription start site (TSS) of RNU1 were rare (1.4±0.3 molecules per cell for −90/+10 RNA), indicating that upstream transcription is negligible (Supplementary Fig. 2c).
They are proposed to consist of a 42 kDa spinin-specific protein with an estimated content of about 2.5 × l05 spinin molecules per cell, which constitutes a substantial portion of the total protein of a spinae-producing bacterium17,19.
In cells expressing no miR-122 decoy, both WT and G28A Jc1 replicated to ~2 × 103 HCV RNA molecules per cell, which concurs with previous models and measurements of HCV steady-state replication in Huh-7.5 cells16.
Quantitative analysis was used to determine that HL60s experiencing transient electroporation internalized between 50 and 125 million nucleic acid-bound PI molecules per cell.
Quantitative flow cytometric analysis revealed GPNMB cell surface molecular density of 1.1 x 10(4) to 7.8 x 10(4) molecules per cell, levels sufficient for mAb targeting.
The unperturbed Sln1 signal transduction system functions with 656 and 6780 molecules per cell of Sln1 kinase and the HOG1 transducer, respectively (Ghaemmaghami et al. 2003).
