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We introduce a computational method for classification of individual DNA molecules measured by an α-hemolysin channel detector.
An in silico model predicting drug permeability is described, which is built based on a large permeability dataset of 7488 compound entries or 5435 structurally unique molecules measured by the same lab using parallel artificial membrane permeability assay (PAMPA).
Historically, the laboratory confirmation of the diagnosis OI rested on cultured dermal fibroblasts to identify decreased or abnormal production of abnormal type I (pro collagen molecules, measured by gel electrophoresis.
Analysis and quantification of the rate of subunit exchange reactions between WT and FT2·WT TTR and between V122I and FT2·V122I TTR; stabilization of V122I TTR and FT2·V122I TTR by small molecules measured by subunit exchange.
These peptides were evaluated experimentally for their binding affinity to the relevant HLA molecules, measured by MHC ELISA as the peptide-dependent MHC rescue after the UV light-mediated exchange of a conditional ligand [ 23– 25].
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Cell-surface molecules were measured by flow cytometry.
Hydrodynamic radii (Rh) of PEG molecules were measured by dynamic light scattering (DLS) (Nanosizer ZS, Malvern Instruments, Worcestershire, UK) at room temperature (25°C).
The remnant ECM molecules were measured by digestion of conditioned cartilage with papain, followed by measuring the remaining uronic acid.
Six hours following activation with TNFα (25 ng/ml), expression of endothelial cell adhesion molecules was measured by flow cytometry.
The induction of potent chemoattractive mediators (chemokines) and neutrophil adhesion molecules were measured by real-time PCR, flow cytometry, and protein assays.
Binding activities of antibodies to the surface of ECs and FLRT2 molecules were measured by using FACSCalibur and FACSCanto II (Becton Dickinson, Franklin Lakes, NJ, USA) [ 17], and the data were analyzed with FlowJo Software Tree Starr, Ashland, OR, USA).
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