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The interaction between membrane-bound hydrophobic compounds and free hydrophilic molecules is monitored in real-time and with high sensitivity and selectivity by combined SPS/SPFS.
The system is designed such that a sample is taken from the fermentor, the metabolism of microbial cells is instantaneously arrested, the cells are lysed in order to release intracellular compounds and in a subsequent binding assay the content of target molecules is monitored.
The flux of molecules is monitored to retain folding intermediates and target misfolded molecules to ER-associated degradation (ERAD) pathways.
Although there are a few reports in which the ubiquitination status of surface molecules is monitored by using surface biotinylation, they only demonstrated that ubiquitinated target molecules are present at the plasma membrane [19], [20].
After pre-binding to microtubules, release of dynein molecules is monitored after addition of 5 mM ATP, with and without 300 nM Lis1.
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Additionally, a drastic increase in solution viscosity due to the formation of giant molecules was monitored during polymerization.
The geometric motion of TEA molecules were monitored in real time across this patterned c(4 × 2) surface.
Upon mitogenic activation, secreted IFN-γ and TNF-α molecules were monitored by performing square wave voltammetry (SWV) at different time points at individually addressable electrodes.
The change in the length of individual ssDNA molecules was monitored by the position change of the bead linked to the ssDNA.
The relative levels of these four molecules were monitored at each cycle of the SELEX procedure to verify and standardize the growth conditions of the cells.
KIF1C molecules were monitored using fluorescently labeled antibody (Video 1).
Related(20)
species is monitored
medications is monitored
models is monitored
genes is monitored
cores is monitored
particles is monitored
composites is monitored
molecules is screened
molecules is restricted
molecules is shown
molecules is presented
molecules is needed
molecules is described
molecules is measured
molecules is discussed
molecules is overstated
molecules is linked
molecules is converted
molecules is immobilized
molecules is released
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