Sentence examples for molecule level we from inspiring English sources

To work with biological motors and DNA at the single molecule level, we develop and utilize state-of-the-art (and often one-of-a-kind) instruments spanning optical trapping, magnetic tweezers, and nanophotonics.

Having established that hybridization enrichment could recover insertions at the single DNA molecule level, we screened 576 µg sperm gDNA from donor B for de novo L1 insertions.

DOI: http://dx.doi.org/10.7554/eLife.04489.003 To study Kar3 motors at the single molecule level, we developed a protocol to express and purify full-length kinesin-14 heterodimers from Saccharomyces cerevisiae using affinity tagged Cik1 and Kar3 fused COOH-terminally to a HaloTag that served as covalent attachment site for the fluorescent dye tetramethylrhodamine (TMR).

To test whether DEASH could recover full-length L1 insertions at the single DNA molecule level, we mixed 24 pg sperm gDNA from donor A containing ∼2 amplifiable molecules of the AL121819 insertion (see above) with 0.5 µg of sperm gDNA from donor B, who lacks the AL121819 insertion.

To assess the motile activity of DNA-kinesin at the single molecule level, we first observed the parallel type that replaced the coiled-coil of native kinesin with DNA at connect position 342, the C-terminal end of the neck linker.

Because long PCR can efficiently amplify regions of 10 kb or more at the single DNA molecule level, we chose 5-kb long insertion targets; if such a target acquired a full-length 6 kb L1 insertion, then the resulting 11-kb DNA fragment would still be amplifiable and could be subsequently purified by hybridization enrichment using L1 specific probes (Fig. 1B).

To study S1 at a single-molecule level, we designed nanoparticle conjugates making use of the strong biotin-streptavidin interaction.

Our study opens an avenue to utilizing force spectroscopy techniques to probe the folding dynamics of proteins in the presence of cofactors at the single-molecule level, and we anticipated that this method can be used to study a wide variety of proteins requiring cofactors for their function.

Rather than seeking differential genes at an individual-molecule level, here we propose a novel method for identifying network biomarkers based on protein-protein interaction affinity (PPIA), which identify the differential interactions at a network level.

We tracked hydroxy-Pdot movement at the single molecule level over time, fostered by the stable nonblinking Pdot emission.

In this paper, we introduce a FRET-based experimental tool to study DNA looping at the single molecule level.