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Two antibodies showed specific bands in the low molecular weight area of mouse serum samples.
We found that DRα bands did not correspond to a species of uniform size but were almost evenly distributed all across the high molecular weight area of the gradient (Fig. 4B).
Before immunodepletion all samples were dominated by the presence of high abundance plasma proteins with albumin as the most marked peak with retention time of 30.5 minutes (Figure 4A), but tumor fluid also presented with a higher signal compared with ascites and plasma showing that there were more proteins eluting in the low molecular weight area after albumin.
Only a few gliadin bands in B. distachyon were present in the lower molecular weight area on SDS-PAGE gels as shown in Figure 1d.
Although not exactly comparable with our experimental set-up, they also found peptides in the low molecular weight area (estimated at 5 kDa) on electrophoresis that had no IgE-binding [ 11].
Furthermore, the lectin blot analysis reveals an increased staining intensity with MAA, in the range of 125 kDa, suggesting that molecules in this molecular weight area are more α2,3-sialylated in the metastatic C4-2B cells.
Similar(52)
The bacterial cellulose showed more carbonyl groups in the low-molecular weight area than expected from the number of reducing ends in this region from the pure cellulose part.
In Cox15 sm/sm samples, the intensity of the band corresponding to individual CIV holocomplex was markedly reduced, and hardly any reaction was detected in the high-molecular-weight area harboring sc.
As expected, more ubiquitin was present in the high molecular weight region above the 136 kDa area where ubiquitinated PER1 had been detected by immunoblotting (Table 2).
These vector entries may be physicochemical properties, for example, molecular weight, surface area, orbital energies, or they may describe topological indices that encode features such as the properties of individual atoms and bonds.
A selection scheme based on the GOLD scores, the Catalyst fit and shape values, and the size of the compounds (molecular weight, surface area, and number of rotatable bonds) was developed and thirteen compounds representing all four chemical classes were selected for experimental studies.
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