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Due to the genetic homogeneity of the genus Brucella, the development of molecular typing tools has been difficult.
With the advent of molecular typing tools, should the traditional classification of relapsing fever spirochetes be readdressed?
With less than ten scaffolds per genome [GenBank:ATNN00000000, GenBank: ATNO00000000, GenBank: ATNP00000000, GenBank: ATNQ00000000], these draft genomes are sufficiently well assembled to allow gene discovery and gene functional analyses and to develop molecular typing tools.
Two patients with M. abscessus otomastoiditis are siblings (patients 5 and 6); a clonal relationship between the causative bacteria is possible and should be investigated by molecular typing tools.
The knowledge derived from this work is extremely useful for future outbreak investigations, not only due to the specific genomic information that facilitates detailed comparisons between different bacterial isolates, but also because the data can be used to add unprecedented resolving power to current molecular typing tools.
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In this study, we evaluated variable number tandem repeats (VNTR) as a molecular typing tool for assessing the genetic diversity within pathovars of X. arboricola.
The MLVA scheme described in this study is a rapid and reliable molecular typing tool that can be used for further epidemiological studies of bacterial diseases caused by X. arboricola pathovars.
Multiple-locus variable number tandem repeat analysis (MLVA) may provide an alternative high throughput molecular typing tool with high epidemiological resolution.
Refined molecular typing tool like FAFLP potentially presents as a highly sensitive rapid technique for strain typing.
A fast, highly sensitive, and specific molecular typing tool is essential for clinical diagnosis and medical treatment.
An increasingly applied molecular typing tool for discrimination of bacterial species with stable clonal population structures is multiple locus variable-number tandem repeat analysis (MLVA).
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