The data shown here render the pheromone receptor genes in Hypocrea an excellent marker to distinguish individuals on strain level, which could be used as a molecular identification tool for closely related strains.
The results from this study stress the importance of the use of molecular identifications tools to describe the occurrence of Trichoderma diversity from this region.
There is a dire need for rapid real-time high-throughput genomic mapping and molecular marker identification tool for isolation of quantitative trait loci, and thereby designing crops with stress, insect, and drought tolerance [7].
Sequencing of D2 subunit spanning 28S rDNA of isolated fungi had been completed for molecular identification imposing bioinformatic tools and among 15 isolates compared where a correction of 100% identification was observed with isolate of B. bassiana strain CCFC225554.
Therefore, with the advent of molecular methods and identification tools based on sequence analysis of multiple genes, it is now possible to identify every Trichoderma isolate and recognize it as a putative new species [ 9, 14, 15].
Therefore, molecular identification is a helpful tool in resolving taxonomic problems in cases where the morphological "boundaries" among monogenean genera or species groups are ambiguous [ 27, 61, 62].
Molecular identification serves as an important tool for conservation [67], consumer advocacy [102], and human health [103].
The mPCR/RLB assay appeared to be a useful alternative tool for the molecular identification of common pathogens.
The 16S rRNA gene is the most frequent target used in clinical laboratories for molecular identification, but in some cases this tool can only help to identify the genus and it is not discriminative enough for speciation within the genus.
