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Using molecular techniques, we have identified the likely translational target of these alcohols in C. albicans as the eukaryotic translation initiation factor 2B (eIF2B).
Following validation by additional molecular techniques, we have characterized these novel candidate regions containing 24 different genes including alterations in two previously reported regions of chromosome 7 associated with the ASD phenotype.
With the use of sophisticated imaging and molecular techniques, we are now able to present the first comprehensive karyotype for switchgrass that quantitatively distinguishes each of the nine base chromosomes of this bioenergy crop.
On the basis of a combination of conventional virus isolation system and molecular techniques, we found that 52.1% (25/48) of patients with SARS admitted to our hospital had HMPV infection.
With the advent of new molecular techniques, we have been able to investigate bacterial diversity in different microhabitats using molecular fingerprinting methods and sequence analysis of microbial small subunit ribosomal (r) RNA genes (16S rRNA) and other universal targets (such as cpn60) [ 13, 36, 37].
After detecting A. vaginae using molecular techniques, we reviewed our culture results to determine if any of our isolates had been identified as G. morbillorum, based on the report by Geissdorfer et al. [ 34] that their A. vaginae isolate was originally misclassified as this organism by the API ID32A system.
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By either immunohistochemistry or molecular technique we found no correlation between DMBT1 expression and tumor histotype, grade, hormonal receptors status, proliferation index, Her2 Neu expression or lymph-nodal metastases.
The powerful molecular techniques that we used to isolate genes expressed by early fetal chorionic villi will lead us to a better understanding of the genetic control of normal human reproduction.
"I thought that with the development of molecular biological techniques, we could ask and answer important questions in the biology of aging, which researchers couldn't [answer] up to that time".
Using the secondary molecular genotyping techniques, we showed that all six isolates were different.
Using standard molecular biology techniques, we prepared a DNA library that encodes Prb with eight randomized binding face residues (shown in Figure 1C).
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