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The use of HPLC-SEC in studies of polypeptides of a molecular mass less than 5000 Da is possible with a stationary phase used in column BioSep-SEC-S2000 BioSep-SEC-S2000 BioSep-SEC-S2000 [19] in thatmolenablesmass rangexcellentfew hundresolutionew thousand Da.
(A) HPLC chromatograms of yam dioscorin peptic hydrolysates with molecular mass between 3 kD and 10 kD, (B) HPLC chromatograms of yam dioscorin peptic hydrolysates with molecular mass less than 3 kD, and (C) DPP IV inhibitory activities of two fractions (5 mg/ml) of dioscorin peptic hydrolysates from molecular cut-off membrane devices, and sitagliptin phosphate was acted as positive controls.
However proteins with molecular mass less than 60 kDa, which is the threshold for glomerular filtration, will be subject to quick renal clearance resulting in shorter half-lives.
Unlike OC125, the majority of 130-22/145-9 associatedas associated with a much lower molecular mass (less than 200 kDa), indicating that a lower molecular mass immunoreactive determination may be a unique CA130 antigenic determinant within CA125 molecule.
The molecular profiles obtained using the TeSeE sheep/goat Western immunoblotting kit (Bio-Rad, Marnes-la-Coquette, France) on a fresh sample of the caudal brainstem from both 05/377 and 06/008 were unlike BSE and classical scrapie but similar to Nor98 with a band of molecular mass less than 15 kDa (fig. 2).
Similar(55)
This well-known rule postulates that drug-like compounds can be most often characterized as having a molecular mass of less than 500 Daltons, fewer than 5 hydrogen bond donors, fewer than 10 hydrogen bond acceptors, and a logP value between -5 and 5.
The majority of these non-protein molecules have a molecular mass of less than 300 Da.
All we can say about it is that it is a serine-protease-sensitive membrane protein with a molecular mass of less than 30 kDa.
In vitro experiments performed with trypanosomes and purified peripheral blood mononucleated cells revealed the existence of a lymphotoxin embedded in the T. evansi membrane: a protein sensitive to serine proteases, with a molecular mass of less than 30 kDa.
Of the 24 mature honeybee CPs, 21 (87.5%) have a molecular mass of less than 30 kDa.
MTs represent a family of ubiquitous, low-molecular-weight, metal-binding proteins that have a molecular mass of less than 7,000 Daltons, containing approximately 33-percent cysteine residues [ 94].
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