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These groups encompassed a variety of molecular functions with no significant deviation from the composition of the overall dataset (Additional file 9).

Molecular function analysis revealed human secreted proteins had 39 types of molecular functions with a total of 3,059 GO IDs.

Tests for significant categorical enrichment were performed using the rank biserial correlation on biological processes and molecular functions with at least ten genes and pathway components with at least five genes measured in our study.

When A. thaliana proteins were annotated with Gene Ontology (GO) terms, paralogous proteins with non-conserved disordered segments were associated with 18 biological processes and 10 molecular functions with corrected p-values < 10E-5.

It is notable that while the individual sites reported to be ageing associated differ to some extent between studies, the results regarding their locations in the genome and the molecular functions with which they are associated are more uniform.

To further understand the potential mechanism at the transcriptional level, we compared gene expression between 48A9 cells and ground control Caski cells with suppression subtractive hybridization (SSH) and reverse Northern blotting methods, and analyzed the relative gene network and molecular functions with the Ingenuity Pathways Analysis (IPA) program.

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A total of 156 DEGs were assigned to three gene ontology classes: biological process, cellular component and molecular function, with 509 functional terms.

In a second assessment of model performance, we showed that GCI annotation levels correlated well with Gene Ontology terms of molecular function, with no correlations observed for the biological process and cellular component categories.

The table ranks the gene enrichment in biological processes, cellular component and molecular function with corresponding GO IDs.

The following GO terms were selected: GOTERM_BP_ALL (biological process), GOTERM_CC_ALL (cellular component) and GOTERM_MF_ALL (molecular function), with classification strategy 'high'high

In this analysis we investigated two major GO categories where 1) biological processes with 30 subgroups and 2) molecular function with 20 subgroups were identified (p < 0.05).

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