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Exact(5)
The combined molecular data matrix included 1,651 aligned positions, with 292 parsimony informative characters.
The final molecular data matrix comprised 195 taxa representing 185 mycalesine species and 10 related taxa as outgroups.
Across six genes, the molecular data matrix consisted of 5352 aligned characters, of which 2839 were variable and 2259 were parsimony informative (Table 3).
Similar analyses of a reduced molecular data matrix (3,703 nucleotides, loops and mt third positions excluded) resulted in a more poorly resolved and supported tree (Fig. 6).
Whereas this scenario indeed receives strong support from the two coding regions (rbcL-gene, atpA-gene), the molecular data matrix also yields a strong conflicting signal from three relatively conserved spacer sequences (i.e. atpB- rbcL, rbcL- accD, and trnL- trnF) that indicates an alternative root placement between Leptopteris– Todea and the remaining Osmunda s.l.l
Similar(55)
Maximum likelihood (ML [ 94]) analyses of nucleotide and microsatellite characters from each of the molecular data matrices were performed.
In the present study, we apply such functions to compute distances between plant individuals as operational taxonomic units (as the hosts) from molecular data matrices that exhibit significant intra-taxon sequence heterogeneity reflected by cloned ITS data (as the associates).
The ILD metrics for all analyses are summarized in Additional file 7. Combined unconstrained analyses of the molecular data and matrix A yielded a single MPT with 10451 steps, CI= 0.432, RI= 0.694 (Fig. 8A).
For this purpose our results were compared with analysis combining the molecular data with a matrix produced applying Shultz's [ 22] character statements to the sampled taxa.
Total evidence analysis, where fossils are placed as terminal taxa in the dating analysis using both molecular and morphological data matrix, could help in exploring the robustness of fossil placement [ 85], but as pointed above, the lack of characters in the Solanaceae seed fossils does not currently permit such analyses.
The PartitionFinder estimated best-fit partitioning schemes and models of molecular evolution of Mycalesina data matrix used in the RaxML tree estimation analysis.
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