Sentence examples for molecular beacon with from inspiring English sources

For a rapid and simple assay, we attempted to use the molecular beacon with real-time PCR and in situ fluorescence imaging.

Further, photoinduced PMHT NB effectively inhibited the unwinding of a molecular beacon with a hairpin structure, indicating that synthetic photoreactive MPC polymers photoregulated the unwinding of DNA.

Here we designed a molecular beacon with a restriction endonuclease recognition site at its loop structure, to study the behavior of the MB on solid matrix and to improve its performance.

We demonstrated that, using a combination of Oct-4 mRNA-targeting molecular beacon with SSEA-1 antibody in flow cytometric analysis, undifferentiated stem cells can be clearly distinguished from differentiated cells.

The introduction of single molecule detection for the interaction of a molecular beacon with DNA leads to an increase in sensitivity from about 10−7 M to 10−11 to 10−12 M (Knemeyer and Sauer 2000).

Isolates from those with clinical infection were tested for the presence of the Panton-Valentine leukocidin (PVL) genes by polymerase chain reaction (PCR) and by molecular beacon with the lukF component of pvl.

The use of molecular beacons with other amplification schemes has been reliably demonstrated, though PCR remains the method of choice.

However, the usual dual-labeled probes do not possess adequate fluorescence quenching ability and sufficient specificity, and molecular beacons with the necessary stem structures are hard to design.

By using molecular beacons with the NASBA amplification a real-time detection system can be generated.

A recent analytical study detected no cross-reactivities with NTM species except some weak cross-hybridization of two rpoB specific molecular beacons with M. malmoense[ 2].

Molecular beacons with DNA (2'-deoxy) backbone labeled with Cy3 fluorophore at the 5' end and Black Hole quencher 2 (BHQ2) at the 3' end were synthesized by MWG Biotech (High Point, North Carolina).