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The inclusion of evolutionarily related genes as modules revealed a striking number of families with expression patterns associated with stemness.
Consideration of the 55 intra-module hub genes from critical regulatory modules revealed a number of signaling and cellular processes were influenced, Figure 5 and Figure S3.
Interestingly, analysis of the functions of genes in these modules revealed a sequential progression of stage-specific core gene networks.
Closer inspection of these modules revealed a transcriptional regulator (SO2426) involved in iron acquisition and ten transcriptional factors involved in anaerobic energy metabolism.
These modules revealed a wide array of functional groups that were overrepresented in gene sets highly correlated with the probes contained in the original signatures.
Gene ontology annotation of the modules revealed a significant overrepresentation in some modules for neuronal processes, e.g., central nervous system development.
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I decided to align this against NVQ Level 2 business admin modules, revealing a recognised standard should students wish to pursue an academic or apprenticeship route.
Anonymous surveys conducted at the conclusions of modules reveal a high degree of satisfaction with the material covered and its perceived benefit to participants (unpublished data).
On the other hand, the OCT1 related module revealed a novel relationship to breast cancer outcome.
Analysis of differential expression on the 3′ expression module revealed a strong effect of sex on gene expression.
A heat map of the Magenta module revealed a distinct pattern of overall decreased expression levels in A/J compared to C57BL/6J.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com