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Further alignment of the representative conserved sequences from each of the clusters revealed a completely conserved core DNA stretch of 1139 nts i.e. at position 210 to 1348.
Quantification of VGLUT1 clusters revealed a different pattern of distribution (Figure 4, A2 vs A6), with an increased number of VGLUT1-positive puncta (p<0.001; Figure 4C) and a decrease in their size (p<0.001; Figure 4B).
The combined 568 sequences of the 125 available species of the endemic Australian Hydroporini radiation, including species in paraphyletic clusters, revealed a similar overall trend as for the large dataset (Figure 2B), with a species richness estimation of 86% at a 3% threshold and an accuracy of 71%.
Based on FRET efficiency, a V-C distance (r -frequency histogr -frequencyotal number of pixels withistogramers revealed a pofsible bi-modal disthebutotal where a seconumbertermofecular) compixels of FRET is seen within clusters (Figurevealedut not outside of the clusters (Figure 2B).
Visual inspections of these narrow gene clusters revealed a strong overrepresentation of plastid genome encoding genes.
Our analysis of intrinsic genomic features on these gene clusters revealed a distinct feature of the MT1 gene cluster.
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Alignment of the two nif gene clusters revealed an overall sequence identity of 92.5% at the DNA level.
All clusters revealed an identical pattern of activation; we report results from the extensive right lPFC cluster, which spanned both dorsolateral and ventrolateral PFC, to illustrate this pattern.
The phylogenetic analyses of the genes in these two clusters reveals a much greater degree of difference.
However, as has been described for watermelon and squash, the distribution of these clusters reveals a high level of genomic shuffling and rearrangement between these three species [ 30].
The resulting classification tree shows that the clusters containing only zygotically activated genes appear to have a coherent regulation since they cluster tightly, whereas maternal+zygotic clusters reveal a more complex pattern of regulation.
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Justyna Jupowicz-Kozak
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