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Physiological measurement Pupil diameter modifications were assessed by eye-tracking (SMI ETG 1); heart, respiratory rate and thoracic volume variations were measured with a dedicated device (Hexoskin).

Pupil diameter modifications were assessed using an eye-tracking system (SMI ETG 1, SensoMotoric Instruments GmbH, Teltow, Germany) (Additional file 1: Fig. S1); heart and respiratory rate and thoracic volume variations were measured using a biometric belt (Hexoskin, Montréal, Canada).

Cell-specific microRNAs, Jumonji histone demethylase gene expression and histone modifications were assessed.

Charge and amino acid modifications were assessed using cation exchange chromatography (CEX) and peptide mapping using reversed-phase (RP HPLCC.

Lifestyle modifications were assessed on self-reported salt intake, physical activity, smoking and alcohol use, according to the Chinese guidelines for prevention and treatment of hypertension [ 15].

Histone modifications were assessed on 132 tumour tissues and 50 normal tissues included in the validation cohort of the LINE-1 study and present on a tissue microarray (described below).

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These modifications are assessed using selected tests of NEPTUN-LWHCR and FLORESTAN experiments.

The structural modifications are assessed by grazing incidence X-ray diffraction (GIXRD) and Fourier transform infra-red spectroscopy (FT-IR).

The ability of the FXR1 and FXR2 paralogues to interact with peptides derived from histone tails carrying various post-translational modifications was assessed by fluorescence polarization assay.

Linear trends and effect modification were assessed using Wald tests.

Effect measure modification was assessed between concussion history and covariates.

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