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Protein expression and post-translational modifications were measured with iTRAQ proteomics using ultra high-precision two-dimensional liquid chromatography tandem mass spectrometry.
Gene and protein expression, DNA methylation, and histone modifications were measured in colon and liver of female offspring.
Levels of ~100 proteins/protein modifications were measured in the hippocampus, cerebellum, and cortex of cohorts of adult female and male mice.
Using reverse phase protein arrays, levels of ~100 proteins/protein modifications were measured in the hippocampus, cerebellum, and cortex of female and male controls and their trisomic Dp10 littermates.
(F – J ) Levels of activation-associated (H3K4me2 and me3, H4K8Ac) and repression-associated (H4K20me3, H3K9me3) histone modifications were measured using ChIP-qPCR in HMECs transfected with control siRNAs (black lines) or siRNA against CTCF (grey lines).
Moderate increases in sugars commonly found in cell walls such as cellobiose, xylonic acid and arabinose, which may indicate cell wall modifications, were measured in sugar beet Fe-deficient root tips.
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The reflection characteristics of the nano-TiO2 before and after surface modification were measured by ultraviolet-visible spectrophotometer (UV-vis) with a wavelength range from 190 to 700 nm.
To determine whether KDM5C regulates specific histone modifications in HCC cells, histone modification patterns were measured after modulation of KDM5C expression.
In order to study the reversible nature of plasma surface modification, contact angles were measured for various storage time starting from the time just after the treatment to 2 days.
Membrane-bound hemichromes, indicators of oxidative membrane damage and inducers of RBC membrane modifications producing enhanced phagocytosis [20] [22], were measured in ring stage-infected RBCs of the index patient, individual 2 (brother), and individual 3 (sister), all homozygous for the GR deficiency, and were compared to ring stage-infected normal RBCs.
Plasma lipids (total cholesterol, triglycerides, and HDLc) were measured by modification of the standard Lipid Research Clinics Protocol with enzymatic reagents for lipid determinations (33).
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