Sentence examples for modifications samples were from inspiring English sources

We analysed the swabs for the presence of Bd with Bd-specific primers in rt-PCR following the protocol by Boyle et al. [33] with slight modifications: samples were run in duplicates and when the two PCR-wells retuned inconsistent results, the analysis was repeated.

ChIP-seq libraries were prepared according to Illumina protocols (Part #11257047 Rev A), with modifications: samples were PCR amplified prior to size selection.

DNA was extracted from silica-dried leaves using the DNeasy® 96 plant kit (Qiagen, Hilden, Germany) with two modifications: samples were lysed 2 h at 65°C, and DNA was eluted in 200 μL of buffer AE.

Chromatin immunoprecipitation (ChIP) was performed as previously described (Wohrle et al, 2007) with the following modifications: Samples were sonicated for 30 min with 30-s intervals on power level 'high' with the Diagnode Bioruptor UCD200.

M-SAA3 was determined using a commercial multispecies SAA ELISA kit (Tridelta Development Ltd, Wicklow, Ireland) according to manufacturer's instruction as used in Eckersall et al. [ 26] with minor modifications; samples were diluted to a minimum dilution of 1 50, or 1 500.

DNA was extracted from 5-micron sections of FFPE tissue (four to eight slides per case) using the QIAamp DNA FFPE Tissue Kit (Qiagen) according to the manufacturer's instructions with few modifications (samples were lysed overnight; 10 uL of additional proteinase K was used on the following day; the tissue was allowed to complete lysis for additional one to two hours).

Assays were carried out using the Comet Assay system (Trevigen; Gaithersburg, MD, USA), according to the manufacturer's instructions with a minor modification: samples were treated with RNaseI and stained with propidium iodide.

The assay was performed as previously described with a few modifications, and samples were run in triplicate.

After modification, the samples were extensively rinsed with absolute ethanol and followed by 500 °C annealing treatment for 1 h in air.

In order to increase the density of the surface hydroxyl groups before the actual surface modification, the samples were first submerged in deionized H2O at 100°C for 1 min. Subsequently, the samples were dried at 50°C to removethe liquid water from the surfaces.

The surface modifications in all samples were analyzed by RBS, GD-OES and XPS.