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To observe the temporal change, samples were collected after heating for 30, 60, and 300 s.
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When a culture attained steady state (after 5 volume changes), samples were collected for estimation of dry cell mass, fluoride release and residual 4-FCA.
For platelet identification and glycoprotein IIb/IIIa (GPIIb/IIIa) expression changes samples were stained with 6 μl anti-GPIIb/IIIa PE (phycoerythin -conjugated monoclonal antibody (CD41-phycoerythin -conjugatedulter, Krefeld, Germonoclonal
The structure of the phase-change samples was checked by X-ray diffraction [8].
After the last resin change, the samples were transferred into gelatin capsules with fresh LR White.
Genes with less than 2.5 fold change across samples were eliminated from consideration.
In order to check the compositions calculated from the weight change, selected samples were analyzed by scanning electron microscopy using energy dispersive X-ray spectroscopy (EDS).
Thermal stabilities and weight changes of samples were tested by TGA.
To identify structural changes, some samples were tempered at 600 or 640 °C.
Histological changes in samples were examined by light microscopy.
Fold changes between samples were determined using the ΔΔCt method.
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