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Mitochondrial sequences show moderate differentiation between all islands considered together (Djerba, Lampedusa, and Kerkennah) and North African mainland haplotypes (Table 1: pairwise FST = 0.16073, p = 0.00000), suggesting recent faunal exchange.
Moreover, SNP markers revealed a higher differentiation, pointing out a moderate differentiation between sativa and sylvestris compartments based on Fst value, and at the population level the high number of loci should solve better the genetic relationship among samples.
Our results demonstrated a moderate differentiation between sativa and sylvestris compartments, even though a connection between several samples of both subspecies may be assumed for the occurrence of cross hybridization events among native wild populations and the cultivated accessions.
According to this work, a moderate differentiation between sativa and sylvestris compartments was discovered, due to centuries long separation of two taxa, making it quite impossible to trace the events of V. vinifera domestication.
The finding of moderate differentiation between the Marianne and Cousin museum populations (FST = 0.09) – two of the most geographically separated islands within the Praslin group (Fig. 1) – is consistent with a scenario of limited, but not ubiquitous, historical dispersal between islands.
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However, there was a statistically significant difference among different degrees of histopathological differentiation of NSCLC (P<0.0001 between well and moderate differentiation, P<0.0001 between well and poor differentiation and P=0.0367 between moderate and poor differentiation).
Effects of inbreeding were also detected in CC corex, but the average estimate of FST decreased in relation to the thematic collection, demonstrating a moderate differentiation, although significant, between the groups that were inferred.
Of the 14 candidate SNPs identified by the transcriptome analyses, eight showed high to moderate differentiation (DI > 0.6) between southern (N = 8) and northern Swedish samples (N = 8) when independently validated by Sanger sequencing.
The pairwise Fst values higher than 0.05 among G1, G2 and G3 subpopulations revealed a moderate differentiation and the relatedness between Eastern and sylvestris individuals groups was confirmed by Fst lower value for G2-G3 pairwise.
The entire panel of 1857 accessions exhibited moderate differentiation and some overlap between the temperate and TS germplasm; representative lines from the TS and temperate region significantly differed, e.g., B73 from the temperate and Ki3 from the TS region of Thailand, but the accessions from the adjacent regions did not markedly differ.
Comparisons between the population clusters within Amazonia revealed moderate differentiation among the Amazonian clusters (mean FST = 0.0751 – 0.1813), particularly between Peru and WCA Brazil, Peru and NEA Brazil, and NEA and WCA Brazil (Table 3).
Related(20)
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