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A novel synthetic model substrate was designed consisting of cellulose, starch and urea as carbon and nitrogen source in a ratio mimicking the basic composition of maize silage.
The 2PET model substrate was docked using GOLD 5.1 with its central ester bond constrained between 2.7 and 3.1 Å in the oxyanion hole formed by the main chain NH groups of F62 and M131 (broken yellow lines).
When direct ethanol fermentation from 20 g/l barley β-glucan as a model substrate was performed with the codisplaying strain, the ethanol concentration reached 7.94 g/l after 24 h of fermentation.
reported that the model substrate was a substrate of the porcine POP enzyme [8].
As basis for our work, the adsorption of BASF's Hydrophobin H*Protein B on a hydrophobic model substrate was investigated.
KA306 (Δ rseA /Δ rseP /Δ clpP ) cells harbouring a plasmid encoding the indicated model substrate was transformed with plasmids encoding the indicated mutant forms of RseP-HM and were grown at 30°C in M9-based medium containing 1 mM IPTG and 1 mM cAMP for 3 hr.
Similar(54)
The docked 2PET model substrate is shown in cyan.
KA306 cells harbouring an appropriate combination of plasmids encoding the indicated RseP-HM mutant and the model substrate were grown in M9-based medium containing 1 mM IPTG at 30°C.
Cells harbouring an appropriate combination of plasmids encoding an RseP derivative and a model substrate were grown at 30°C in L medium containing 1 mM IPTG and 1 mM cAMP for 3.5 hr.
Cells harbouring an appropriate combination of plasmids encoding an RseP derivative and a model substrate were grown at 30°C in M9 medium supplemented with 20 μg/ml of each of the 20 amino acids, 2 μg/ml thiamine, 0.4% glucose, 1 mM IPTG and 1 mM cAMP for 3 hr.
The enzymatic hydrolysis of the biodegradable polyester ecoflex and of a variety of oligomeric and polymeric ecoflex model substrates was investigated.
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